Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

Localizing phosphorylation sites by data-independent acquisition (DIA)-based proteomics is still challenging. Here, the authors develop algorithms for phosphosite localization and stoichiometry determination, and incorporate them into single-shot DIA-phosphoproteomics workflows.

Bibliographic Details
Main Authors: Dorte B. Bekker-Jensen, Oliver M. Bernhardt, Alexander Hogrebe, Ana Martinez-Val, Lynn Verbeke, Tejas Gandhi, Christian D. Kelstrup, Lukas Reiter, Jesper V. Olsen
Format: Article
Language:English
Published: Nature Publishing Group 2020-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-14609-1

Internet

https://doi.org/10.1038/s41467-020-14609-1

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