Patterns of PCR Amplification Artifacts of the Fungal Barcode Marker in a Hybrid Mushroom

Patterns of PCR Amplification Artifacts of the Fungal Barcode Marker in a Hybrid Mushroom

The polymerase chain reaction (PCR) is widely used in modern biology and medicine. However, PCR artifacts can complicate the interpretation of PCR-based results. The internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster is the consensus fungal barcode marker and suspected PCR ar...

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Bibliographic Details
Main Authors: Jun-Liang Zhou, Jianping Xu, An-Guo Jiao, Li Yang, Jie Chen, Philippe Callac, Yu Liu, Shou-Xian Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-11-01
Series:Frontiers in Microbiology
Subjects:
polymerase chain reaction
ITS
mutation spectrum
chimera sequence
recombination
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.02686/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.02686/full

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