Seamless assembly of recombinant adenoviral genomes from high-copy plasmids.

The adenoviruses are essential tools for basic research and therapeutic development. Robust methods for the generation of mutant and recombinant viruses are crucial for these diverse applications. Here we describe a simple plasmid-based method that permits highly efficient modification of the adenov...

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Bibliographic Details
Main Authors: Jessica J Miciak, Jason Hirshberg, Fred Bunz
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6021080?pdf=render