Manipulation of TAD reorganization by chemical-dependent genome linking

Manipulation of TAD reorganization by chemical-dependent genome linking

Summary: Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence...

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Bibliographic Details
Main Authors: Jia Wang, Qian Ma, Peihang Fang, Qi Tian, Haopeng Yu, Junyi Sun, Junjun Ding
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Developmental biology
Genomics
MolecularBiology
CRISPR
Stem Cells
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005050
Description
Summary:Summary: Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell fate transitions. The advantages are that the manipulation does not change the genome and is reversible by withdrawing ABA. The major challenge is how to select linking loci for efficient TAD reorganization.For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).
ISSN:2666-1667

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