Pol II-expressed shRNA knocks down Sod2 gene expression and causes phenotypes of the gene knockout in mice.
RNA interference (RNAi) has been used increasingly for reverse genetics in invertebrates and mammalian cells, and has the potential to become an alternative to gene knockout technology in mammals. Thus far, only RNA polymerase III (Pol III)-expressed short hairpin RNA (shRNA) has been used to make s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2006-01-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC1358942?pdf=render |