The Effect of Divalent Metal Ions on the Temperature Stability of Poly(I:C) Duplex

The effect of divalent ions on the thermal stability of the double-stranded polynucleotide polyinosinic:polycytidylic acid (poly(I:C)) in a buffer solution (pH 7) was studied by UV absorption spectroscopy and thermal denaturation by measuring the absorbance at 260 nm. The concentration dependence of...

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Bibliographic Details
Main Authors: Evgeniya Usenko, Vladimir Valeev, Alexander Glamazda, Victor Karachevtsev
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Journal of Spectroscopy
Online Access:http://dx.doi.org/10.1155/2020/8850214