Improved purification process of β- and α-trypsin isoforms by ion-exchange chromatography

Improved purification process of β- and α-trypsin isoforms by ion-exchange chromatography

The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography i...

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Bibliographic Details
Main Authors: Alexandre Martins Costa Santos, Jamil Silvano de Oliveira, Eustáquio Resende Bittar, Anderson Lourenço da Silva, Marcos Luiz dos Mares Guia, Marcelo Porto Bemquerer, Marcelo Matos Santoro
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2008-08-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Trypsin isoforms
resolution
ion-exchange chromatography
purification
specific activity
mass spectrometry
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009

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