In vitro DNA SCRaMbLE

SCRaMbLE allows for the rapid and large scale rearrangement of genetic data in yeast carrying synthetic chromosomes. Here the authors demonstrate an in vitro use of the method to generate DNA libraries for optimization of biochemical reactions.

Bibliographic Details
Main Authors: Yi Wu, Rui-Ying Zhu, Leslie A. Mitchell, Lu Ma, Rui Liu, Meng Zhao, Bin Jia, Hui Xu, Yun-Xiang Li, Zu-Ming Yang, Yuan Ma, Xia Li, Hong Liu, Duo Liu, Wen-Hai Xiao, Xiao Zhou, Bing-Zhi Li, Ying-Jin Yuan, Jef D. Boeke
Format: Article
Language:English
Published: Nature Publishing Group 2018-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03743-6