A new chromatographic system for vitamin D3, and its metabolites: resolution of a new vitamin D3, metabolite

A simple yet powerful new chromatographic procedure for vitamin D3 and its metabolites is described. Liquid–gel partition chromatography on Sephadex LH-20 using a solvent of various percentages of CHCl3 in Skellysolve B (petroleum ether, bp 67–69°C) permits excellent resolution of vitamin D3, 25-hyd...

Full description

Bibliographic Details
Main Authors: M.F. HOLICK, H.F. DeLUCA
Format: Article
Language:English
Published: Elsevier 1971-07-01
Series:Journal of Lipid Research
Subjects:
rat
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520394955