Structural characterization of recombinant streptokinase following recovery from inclusion bodies using different chemical solubilization treatments

Circular dichroism (CD) in far-UV region was employed to study the extent of changes occurred in the secondary structures of recombinant streptokinase (rSK), solubilized from inclusion bodies (IBs) by different chemicals and refolded/purified by chromatographic techniques. The secondary structure di...

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Bibliographic Details
Main Authors: Khadijeh Babaei Sheli, Masoud Ghorbani, Azadeh Hekmat, Bita Soltanian, Alireza Mohammadian, Reza Jalalirad
Format: Article
Language:English
Published: Elsevier 2018-09-01
Series:Biotechnology Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X1730348X