Using the dCas9-KRAB system to repress gene expression in hiPSC-derived NGN2 neurons

Using the dCas9-KRAB system to repress gene expression in hiPSC-derived NGN2 neurons

Summary: We describe a CRISPR inhibition (CRISPRi) protocol to repress endogenous gene expression (e.g., ATP6V1A) in human induced pluripotent stem cell-derived NGN2-induced glutamatergic neurons. CRISPRi enables efficient and precise gene repression of one or multiple target genes via delivering gR...

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Bibliographic Details
Main Authors: Aiqun Li, Samuel Cartwright, Alex Yu, Seok-Man Ho, Nadine Schrode, P.J. Michael Deans, Marliette R. Matos, Meilin Fernandez Garcia, Kayla G. Townsley, Bin Zhang, Kristen J. Brennand
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Molecular Biology
CRISPR
Neuroscience
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002872

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http://www.sciencedirect.com/science/article/pii/S2666166721002872

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