Biotinylation of low density lipoproteins via free amino groups without loss of receptor binding activity.
Low density lipoproteins were biotinylated via free amino groups using carbodiimide-activated biotin or D-biotin-N-hydroxysuccinimide ester. The receptor binding activity of the biotinylated LDL was determined by their ability to displace 125I-labeled LDL from the rat hepatic LDL receptor in the lip...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1987-12-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520385837 |