Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization
Abstract Background In situ analysis of biomarkers such as DNA, RNA and proteins are important for research and diagnostic purposes. At the RNA level, plant gene expression studies rely on qPCR, RNAseq and probe-based in situ hybridization (ISH). However, for ISH experiments poor stability of RNA an...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2020-05-01
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Series: | Plant Methods |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13007-020-00614-4 |