Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization

Abstract Background In situ analysis of biomarkers such as DNA, RNA and proteins are important for research and diagnostic purposes. At the RNA level, plant gene expression studies rely on qPCR, RNAseq and probe-based in situ hybridization (ISH). However, for ISH experiments poor stability of RNA an...

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Bibliographic Details
Main Authors: Shyam Solanki, Gazala Ameen, Jin Zhao, Jordan Flaten, Pawel Borowicz, Robert S. Brueggeman
Format: Article
Language:English
Published: BMC 2020-05-01
Series:Plant Methods
Subjects:
RNA
Online Access:http://link.springer.com/article/10.1186/s13007-020-00614-4