Tuning the drug efflux activity of an ABC transporter in vivo by in vitro selected DARPin binders.

Tuning the drug efflux activity of an ABC transporter in vivo by in vitro selected DARPin binders.

ABC transporters use the energy from binding and hydrolysis of ATP to import or extrude substrates across the membrane. Using ribosome display, we raised designed ankyrin repeat proteins (DARPins) against detergent solubilized LmrCD, a heterodimeric multidrug ABC exporter from Lactococcus lactis. Se...

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Bibliographic Details
Main Authors: Markus A Seeger, Anshumali Mittal, Saroj Velamakanni, Michael Hohl, Stefan Schauer, Ihsene Salaa, Markus G Grütter, Hendrik W van Veen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3366976?pdf=render

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http://europepmc.org/articles/PMC3366976?pdf=render

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