Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming

Summary: Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to so...

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Bibliographic Details
Main Authors: Zhongfu Ying, Zihuang Liu, Ge Xiang, Yanmin Xin, Junwei Wang, Xingguo Liu
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002756