Stabilization of the Single-Chain Fragment Variable by an Interdomain Disulfide Bond and Its Effect on Antibody Affinity

Stabilization of the Single-Chain Fragment Variable by an Interdomain Disulfide Bond and Its Effect on Antibody Affinity

The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B1 (AFB1) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody af...

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Bibliographic Details
Main Authors: Wei Chen, Hao Zhang, Yong-Quan Chen, Feng-Wei Tian, Hai-Qin Chen, Zhen-Nan Gu, Lian Yang, Jian-Xin Zhao
Format: Article
Language:English
Published: MDPI AG 2010-12-01
Series:International Journal of Molecular Sciences
Subjects:
aflatoxin
affinity
disulfide bond
single-chain fragment variable
stability
Online Access:http://www.mdpi.com/1422-0067/12/1/1/

Internet

http://www.mdpi.com/1422-0067/12/1/1/

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