Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Abstract Background The methanol-regulated AOX1 promoter (P AOX1 ) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol dependence of P AOX1 restricts its application, it is necessary to...

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Bibliographic Details
Main Authors: Ching-Hsiang Chang, Hao-An Hsiung, Kai-Lin Hong, Ching-Tsan Huang
Format: Article
Language:English
Published: BMC 2018-12-01
Series:BMC Biotechnology
Subjects:
Pichia pastoris
Recombinant protein expression
AOX1 promoter
Methanol
Methanol expression regulator 1
Synthetic gene circuit
Online Access:http://link.springer.com/article/10.1186/s12896-018-0492-4

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http://link.springer.com/article/10.1186/s12896-018-0492-4

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