Quantitative analysis of the dystrophin gene by real-time PCR

Quantitative analysis of the dystrophin gene by real-time PCR

Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine diagnostics of DMD/BMD deletion carriers. Twenty female...

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Bibliographic Details
Main Authors: Maksimovic Nela, Anđelkovic Ana, Milic-Rasic Vedrana, Rakocevic-Stojanovic Vidosava, Kastratovic-Kotlica Biljana, Brankovic S., Damnjanovic Tatjana, Jekic Biljana, Bunjevacki Vera, Lukovic Ljiljana, Perovic Dijana, Cvjeticanin Suzana, Novakovic Ivana
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2012-01-01
Series:Archives of Biological Sciences
Subjects:
Duchenne/Becker muscular dystrophy
Real-time PCR
SYBR® Green
ΔΔCt method
carrier detection
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202787M.pdf

Internet

http://www.doiserbia.nb.rs/img/doi/0354-4664/2012/0354-46641202787M.pdf

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