A novel quantitative fluorescent reporter assay for RAG targets and RAG activity

A novel quantitative fluorescent reporter assay for RAG targets and RAG activity

Recombination-Activating Genes (RAG) 1 and 2 form the site specific recombinase that mediates V(D)J recombination, a process of DNA editing required for lymphocyte development and responsible for their diverse repertoire of antigen receptors. Mistargeted RAG activity associates with genome alteratio...

Full description

Bibliographic Details
Main Authors: Ines eTrancoso, Marie eBonnet, Rui eGardner, Jorge eCarneiro, Vasco M Barreto, Jocelyne eDemengeot, Leonor M Sarmento
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-05-01
Series:Frontiers in Immunology
Subjects:
Green Fluorescent Proteins
Humans
Leukemia
Lymphocytes
Plasmids
Recombination, Genetic
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00110/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00110/full

Similar Items