Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe
Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through homologous recombination. Despite the advanta...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
The Royal Society
2015-01-01
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Series: | Open Biology |
Subjects: | |
Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150054 |