Tobacco etch virus (TEV) protease with multiple mutations to improve solubility and reduce self‐cleavage exhibits enhanced enzymatic activity

Tobacco etch virus (TEV) protease with multiple mutations to improve solubility and reduce self‐cleavage exhibits enhanced enzymatic activity

Tobacco etch virus (TEV) protease is a 27‐kDa catalytic domain of the polyprotein nuclear inclusion a (NIa) in TEV, which recognizes the specific amino acid sequence ENLYFQG/S and cleaves between Q and G/S. Despite its substrate specificity, its use is limited by its autoinactivation through self‐cl...

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Bibliographic Details
Main Authors: Heejin Nam, Beom J. Hwang, Deog‐Young Choi, Sooim Shin, Moonsung Choi
Format: Article
Language:English
Published: Wiley 2020-04-01
Series:FEBS Open Bio
Subjects:
enzyme kinetics
inclusion body
multiple mutations
self‐inactivation
substrate specificity
tobacco etch virus protease
Online Access:https://doi.org/10.1002/2211-5463.12828

Internet

https://doi.org/10.1002/2211-5463.12828

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