Prediction of off-target effects of the CRISPR/Cas9 system for design of sgRNA

CRISPR/Cas9 genome editing technology is the frontier of life science research. They have been used to cure human genetic diseases, achieve cell personalized treatment, develop new drugs, and improve the genetic characteristics of crops and other fields. This system relies on the enzyme Cas9 cutting...

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Bibliographic Details
Main Authors: Guo Calvin, Zhen David
Format: Article
Language:English
Published: EDP Sciences 2020-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/45/e3sconf_iceeb2020_04018.pdf