A method for rapidly screening functionality of actin mutants and tagged actins

<p>Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidly screen the folding capacity and functionality of...

Full description

Bibliographic Details
Main Authors: Rommelaere Heidi, Waterschoot Davy, Neirynck Katrien, Vandekerckhove Jo&euml;l, Ampe Christophe
Format: Article
Language:English
Published: BMC 2004-01-01
Series:Biological Procedures Online
Subjects:
Online Access:http://www.biologicalprocedures.com/bpo/arts/1/94/m94.pdf