Improving Protein Crystal Quality by the Without-Oil Microbatch Method: Crystallization and Preliminary X-ray Diffraction Analysis of Glutathione Synthetase from Pseudoalteromonas haloplanktis

Improving Protein Crystal Quality by the Without-Oil Microbatch Method: Crystallization and Preliminary X-ray Diffraction Analysis of Glutathione Synthetase from Pseudoalteromonas haloplanktis

Glutathione synthetases catalyze the ATP-dependent synthesis of glutathione from L-γ-glutamyl-L-cysteine and glycine. Although these enzymes have been sequenced and characterized from a variety of biological sources, their exact catalytic mechanism is not fully understood and nothing is known about...

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Bibliographic Details
Main Authors: Antonella Albino, Emmanuele De Vendittis, Filomena Sica, Mariorosario Masullo, Irene Russo Krauss, Antonello Merlino, Alessandro Vergara, Andrea Pica
Format: Article
Language:English
Published: MDPI AG 2011-09-01
Series:International Journal of Molecular Sciences
Subjects:
crystal quality
without-oil microbatch
glutathione synthetase
psychrophile
X-ray crystallography
Online Access:http://www.mdpi.com/1422-0067/12/9/6312/

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http://www.mdpi.com/1422-0067/12/9/6312/

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