Efficient Stereospecific Hβ2/3 NMR Assignment Strategy for Mid-Size Proteins

Efficient Stereospecific Hβ2/3 NMR Assignment Strategy for Mid-Size Proteins

We present a strategy for stereospecific NMR assignment of Hβ2 and Hβ3 protons in mid-size proteins (~150 residues). For such proteins, resonance overlap in standard experiments is severe, thereby preventing unambiguous assignment of a large fraction of β-methylenes. To al...

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Bibliographic Details
Main Authors: Alexandra Born, Morkos A. Henen, Parker Nichols, Jing Wang, David N. Jones, Beat Vögeli
Format: Article
Language:English
Published: MDPI AG 2018-06-01
Series:Magnetochemistry
Subjects:
NMR spectroscopy
biological macromolecules
structure calculation
stereospecific assignment
J-coupling
proteins
Online Access:http://www.mdpi.com/2312-7481/4/2/25

Internet

http://www.mdpi.com/2312-7481/4/2/25

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