Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data

Abstract Background Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Althoug...

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Bibliographic Details
Main Authors: Nelly Olova, Felix Krueger, Simon Andrews, David Oxley, Rebecca V. Berrens, Miguel R. Branco, Wolf Reik
Format: Article
Language:English
Published: BMC 2018-03-01
Series:Genome Biology
Subjects:
WGBS
Biases
Artefacts
Bisulfite conversion
Degradation
DNA methylation
Online Access:http://link.springer.com/article/10.1186/s13059-018-1408-2

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http://link.springer.com/article/10.1186/s13059-018-1408-2

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