Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants

Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious...

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Bibliographic Details
Main Authors: He Yubing, Zhu Min, Wang Lihao, Wu Junhua, Wang Qiaoyan, Wang Rongchen, Zhao Yunde
Format: Article
Language:English
Published: Elsevier 2019-03-01
Series:Rice Science
Online Access:http://www.sciencedirect.com/science/article/pii/S1672630819300058