Decoding Essential Amino Acid Residues in the Substrate Groove of a Non-Specific Nuclease from <i>Pseudomonas syringae</i>

Decoding Essential Amino Acid Residues in the Substrate Groove of a Non-Specific Nuclease from <i>Pseudomonas syringae</i>

Non-specific nucleases (NSN) are of interest for biotechnological applications, including industrial downstream processing of crude protein extracts or cell-sorting approaches in microfabricated channels. Bacterial nucleases belonging to the superfamily of phospholipase D (PLD) are featured for thei...

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Bibliographic Details
Main Authors: Lynn Sophie Schwardmann, Sarah Schmitz, Volker Nölle, Skander Elleuche
Format: Article
Language:English
Published: MDPI AG 2019-11-01
Series:Catalysts
Subjects:
dnase
kinetic profiles
rnase
semi-rational mutagenesis
substrate specificity
Online Access:https://www.mdpi.com/2073-4344/9/11/941

Internet

https://www.mdpi.com/2073-4344/9/11/941

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