Quantifying mRNA and microRNA with qPCR in cervical carcinogenesis: a validation of reference genes to ensure accurate data.

A number of recent studies have catalogued global gene expression patterns in a panel of normal, tumoral cervical tissues so that potential biomarkers can be identified. The qPCR has been one of the most widely used technologies for detecting these potential biomarkers. However, few studies have inv...

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Main Authors: Maria da Conceição Gomes Leitão, Eliane Campos Coimbra, Rita de Cássia Pereira de Lima, Mariléa de Lima Guimarães, Sandra de Andrade Heráclio, Jacinto da Costa Silva Neto, Antonio Carlos de Freitas
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4217744?pdf=render