A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids

Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens, including kinetoplastids. CRISPR technology provides the opportunity to develop scalable methods for high-throughput productio...

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Bibliographic Details
Main Authors: Tom Beneke, Ross Madden, Laura Makin, Jessica Valli, Jack Sunter, Eva Gluenz
Format: Article
Language:English
Published: The Royal Society 2017-01-01
Series:Royal Society Open Science
Subjects:
leishmania
trypanosoma
crispr
genome editing
t7 rna polymerase
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.170095

Internet

https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.170095

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