The construction of bifunctional fusion xylanolytic enzymes and the prediction of optimum reaction conditions for the enzyme activity
Four chimeric xylanolytic enzymes were formed by fusion of a thermally stable xylanase XynCDBFV either to the N-terminus or C-terminus of a thermally stable acetylxylan esterase AxeS20E, with or without a Gly-rich flexible linker (S2). The three-dimensional (3D) structures of the chimeric enzymes we...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
North Carolina State University
2012-11-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://www.ncsu.edu/bioresources/BioRes_07/BioRes_07_4_5647_Pai_WGL_Construction_Bifunc_Xylanolytic_Reaction_Activ_3137.pdf |