A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
Strain engineering is increasingly the bottleneck step in synthetic biology workflows. Here the authors present GTR-CRISPR for rapid, multiplexed engineering of yeast metabolic pathways.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2019-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-09005-3 |