Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells

Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells

Organisms with targeted genomic modifications are efficiently produced by gene editing in embryos using CRISPR/Cas9 RNA-guided DNA endonuclease. Here, to facilitate germline editing in rats, we used CRISPR/Cas9 to catalyze targeted genomic mutations in rat spermatogonial stem cell cultures. CRISPR/C...

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Bibliographic Details
Main Authors: Karen M. Chapman, Gerardo A. Medrano, Priscilla Jaichander, Jaideep Chaudhary, Alexandra E. Waits, Marcelo A. Nobrega, James M. Hotaling, Carole Ober, F. Kent Hamra
Format: Article
Language:English
Published: Elsevier 2015-03-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124715001989

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http://www.sciencedirect.com/science/article/pii/S2211124715001989

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