Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing...

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Bibliographic Details
Main Authors: Duc Long Nguyen, Hanim Kim, Dasom Kim, Jin Oh Lee, Myung Chan Gye, Young-Pil Kim
Format: Article
Language:English
Published: MDPI AG 2018-03-01
Series:Sensors
Subjects:
luciferase
bioluminescence
matrix metalloproteinase
intein
biotinylation
Online Access:http://www.mdpi.com/1424-8220/18/3/875

Internet

http://www.mdpi.com/1424-8220/18/3/875

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