Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9

Abstract Background Targeted DNA integration is widely used in basic research and commercial applications because it eliminates positional effects on transgene expression. Targeted integration in mammalian cells is generally achieved through a double crossover event between the genome and a linear d...

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Bibliographic Details
Main Authors: Xiaofeng Liu, Min Wang, Yufeng Qin, Xuan Shi, Peiqing Cong, Yaosheng Chen, Zuyong He
Format: Article
Language:English
Published: BMC 2018-10-01
Series:BMC Biotechnology
Subjects:
Single crossover
ZFN
CRISPR/Cas9
Knock-in
CCR5
Online Access:http://link.springer.com/article/10.1186/s12896-018-0474-6

Internet

http://link.springer.com/article/10.1186/s12896-018-0474-6

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