Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays

<p>Abstract</p> <p>Background</p> <p>The development of reverse transcription – quantitative real-time PCR (RT-qPCR) platforms that can simultaneously measure the expression of multiple genes is dependent on robust assays that function under identical thermal cycling co...

Full description

Bibliographic Details
Main Authors: Dobrovic Alexander, Mikeska Thomas
Format: Article
Language:English
Published: BMC 2009-06-01
Series:BMC Research Notes
Online Access:http://www.biomedcentral.com/1756-0500/2/112