Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays
<p>Abstract</p> <p>Background</p> <p>The development of reverse transcription – quantitative real-time PCR (RT-qPCR) platforms that can simultaneously measure the expression of multiple genes is dependent on robust assays that function under identical thermal cycling co...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
BMC
2009-06-01
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Series: | BMC Research Notes |
Online Access: | http://www.biomedcentral.com/1756-0500/2/112 |