Optimization of Mature Embryo-Based Tissue Culture and <i>Agrobacterium</i>-Mediated Transformation in Model Grass <i>Brachypodium distachyon</i>

Optimization of Mature Embryo-Based Tissue Culture and <i>Agrobacterium</i>-Mediated Transformation in Model Grass <i>Brachypodium distachyon</i>

<i>Agrobacterium</i>-mediated genetic transformation is well established in the model grass <i>Brachypodium distachyon</i>. However, most protocols employ immature embryos because of their better regenerative capacity. A major problem associated with the immature embryo syste...

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Bibliographic Details
Main Authors: Guangrun Yu, Jianyong Wang, Li Miao, Mengli Xi, Qiongli Wang, Kai Wang
Format: Article
Language:English
Published: MDPI AG 2019-10-01
Series:International Journal of Molecular Sciences
Subjects:
<i>brachypodium distachyon</i>
genetic transformation
mature embryo
seed shearing
photoautotrophic rooting
Online Access:https://www.mdpi.com/1422-0067/20/21/5448

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https://www.mdpi.com/1422-0067/20/21/5448

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