Effects of enucleation method on in vitro and in vivo development rate of cloned pig embryos

Enucleation is a crucial procedure for mammalian somatic cell nuclear transfer (SCNT), especially for domestic animal cloning. Oocytes of domestic animals such as pigs and cattle contain dark lipid droplets that hinder localization and removal of the nucleus. Using an oocyte enucleation technique th...

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Bibliographic Details
Main Author: Chengcheng ZHAO, Junsong SHI, Rong ZHOU, Ranbiao MAI, Lvhua LUO, Xiaoyan HE, Hongmei JI, Gengyuan CAI, Dewu LIU, Enqin ZHENG, Zhenfang WU, Zicong LI
Format: Article
Language:English
Published: Higher Education Press 2019-02-01
Series:Frontiers of Agricultural Science and Engineering
Subjects:
Online Access:http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/22426/1525939972131-1066135325.pdf