Effects of enucleation method on in vitro and in vivo development rate of cloned pig embryos
Enucleation is a crucial procedure for mammalian somatic cell nuclear transfer (SCNT), especially for domestic animal cloning. Oocytes of domestic animals such as pigs and cattle contain dark lipid droplets that hinder localization and removal of the nucleus. Using an oocyte enucleation technique th...
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Format: | Article |
Language: | English |
Published: |
Higher Education Press
2019-02-01
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Series: | Frontiers of Agricultural Science and Engineering |
Subjects: | |
Online Access: | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/22426/1525939972131-1066135325.pdf |