DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR

DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR

The aim of the work was to construct and test the recombinant positive control for F. tularensis detection by a real-time polymerase chain reaction (qPCR). The molecular TA-cloning of pTZ57_F/R plasmid ligated with tul4 gene PCR product into DH5α E. coli. The minimal detection level in qPCR was one...

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Main Authors: O. B. Zlenko, A. P. Gerilovych
Format: Article
Language:English
Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2018-08-01
Series:Biotechnologia Acta
Subjects:
ecombinant positive control
qPCR
tularemia
molecular cloning
Online Access:http://biotechnology.kiev.ua/images/storage/4_2018/zlenko_4_2018.pdf
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spelling doaj-35259dd607b746a9a5252b12e39d782f2020-11-24T21:09:58ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Biotechnologia Acta2410-77512410-776X2018-08-01114687210.15407/biotech11.04.068DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCRO. B. Zlenko0A. P. Gerilovych1National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine” of the National Academy of Agrarian Sciences of Ukraine, KharkivNational Scientific Center “Institute of Experimental and Clinical Veterinary Medicine” of the National Academy of Agrarian Sciences of Ukraine, KharkivThe aim of the work was to construct and test the recombinant positive control for F. tularensis detection by a real-time polymerase chain reaction (qPCR). The molecular TA-cloning of pTZ57_F/R plasmid ligated with tul4 gene PCR product into DH5α E. coli. The minimal detection level in qPCR was one copy number per reaction. The obtained positive control was highly sensitive, specific and safe qPCR in the laboratory tularemia diagnostics.http://biotechnology.kiev.ua/images/storage/4_2018/zlenko_4_2018.pdfecombinant positive controlqPCRtularemiamolecular cloning
collection DOAJ
language English
format Article
sources DOAJ
author O. B. Zlenko
A. P. Gerilovych
spellingShingle O. B. Zlenko
A. P. Gerilovych
DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
Biotechnologia Acta
ecombinant positive control
qPCR
tularemia
molecular cloning
author_facet O. B. Zlenko
A. P. Gerilovych
author_sort O. B. Zlenko
title DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
title_short DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
title_full DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
title_fullStr DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
title_full_unstemmed DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
title_sort development of recombinant positive control for francisella tularensis detection by q-pcr
publisher National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
series Biotechnologia Acta
issn 2410-7751
2410-776X
publishDate 2018-08-01
description The aim of the work was to construct and test the recombinant positive control for F. tularensis detection by a real-time polymerase chain reaction (qPCR). The molecular TA-cloning of pTZ57_F/R plasmid ligated with tul4 gene PCR product into DH5α E. coli. The minimal detection level in qPCR was one copy number per reaction. The obtained positive control was highly sensitive, specific and safe qPCR in the laboratory tularemia diagnostics.
topic ecombinant positive control
qPCR
tularemia
molecular cloning
url http://biotechnology.kiev.ua/images/storage/4_2018/zlenko_4_2018.pdf
work_keys_str_mv AT obzlenko developmentofrecombinantpositivecontrolforfrancisellatularensisdetectionbyqpcr
AT apgerilovych developmentofrecombinantpositivecontrolforfrancisellatularensisdetectionbyqpcr
_version_ 1716756841390669824

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