DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION BY Q-PCR
The aim of the work was to construct and test the recombinant positive control for F. tularensis detection by a real-time polymerase chain reaction (qPCR). The molecular TA-cloning of pTZ57_F/R plasmid ligated with tul4 gene PCR product into DH5α E. coli. The minimal detection level in qPCR was one...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2018-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/4_2018/zlenko_4_2018.pdf |