tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with si...

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Bibliographic Details
Main Authors: Daniel J. Nieves, Geva Hilzenrat, Jason Tran, Zhengmin Yang, Hugh H. MacRae, Matthew A. B. Baker, J. Justin Gooding, Katharina Gaus
Format: Article
Language:English
Published: The Royal Society 2019-12-01
Series:Royal Society Open Science
Subjects:
protein tags
monovalent
covalent
dna-paint imaging
superresolution
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191268

Internet

https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191268

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