Stimulation by serum of 32P incorporation into the phospholipids of Ehrlich ascites cells

Ehrlich ascites cells adapted for suspension culture were incubated with 32PO43- in a Krebs-Ringer-HEPES (N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonate) buffer with or without 5% calf serum. Specific activity was determined for cellular [γ-32P]ATP, total phospholipids, and the individual pho...

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Bibliographic Details
Main Author: P B Schneider
Format: Article
Language:English
Published: Elsevier 1977-03-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520417031