Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design
<p>Abstract</p> <p>Background</p> <p>RNA interference (RNAi) has been one of the most rapidly expanding areas of biological research in the past decade, revolutionizing the ability to analyze gene function. Thorough validation of siRNA duplexes is required prior to use...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2010-03-01
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Series: | BMC Research Notes |
Online Access: | http://www.biomedcentral.com/1756-0500/3/53 |