Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design

<p>Abstract</p> <p>Background</p> <p>RNA interference (RNAi) has been one of the most rapidly expanding areas of biological research in the past decade, revolutionizing the ability to analyze gene function. Thorough validation of siRNA duplexes is required prior to use...

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Bibliographic Details
Main Authors: Chapman Elinor A, Williams Catrin M, Holmes Katherine, Cross Michael J
Format: Article
Language:English
Published: BMC 2010-03-01
Series:BMC Research Notes
Online Access:http://www.biomedcentral.com/1756-0500/3/53