Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride

Abstract Background Long-term in vitro culture of blood stage Plasmodium parasites invariably leads to asynchronous parasite development. The most often used technique to synchronize Plasmodium falciparum culture is sorbitol treatment, which differentially induces osmotic lysis of trophozoite- and s...

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Bibliographic Details
Main Authors: Sutharinee Ngernna, Anongruk Chim-ong, Wanlapa Roobsoong, Jetsumon Sattabongkot, Liwang Cui, Wang Nguitragool
Format: Article
Language:English
Published: BMC 2019-04-01
Series:Malaria Journal
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12936-019-2783-1