Purification and properties of asparaginase from the testa of immature seeds of pea (Pisum sativum L.)

A K+-dependent asparaginase (E.C. 3.5.1.1.) was purified 1328-fold from the testas of immature pea seeds (Pisum sativum L., var. Bolero) and characterized. Antibodies raised against purified asparaginase cross-reacted with the putative asparaginase band in Western blot analyses of semi-purified extr...

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Bibliographic Details
Main Authors: Chagas Eliana P., Sodek Ladaslav
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2001-01-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300004