Genetically Encoded FRET-Sensor Based on Terbium Chelate and Red Fluorescent Protein for Detection of Caspase-3 Activity

Genetically Encoded FRET-Sensor Based on Terbium Chelate and Red Fluorescent Protein for Detection of Caspase-3 Activity

This article describes the genetically encoded caspase-3 FRET-sensor based on the terbium-binding peptide, cleavable linker with caspase-3 recognition site, and red fluorescent protein TagRFP. The engineered construction performs two induction-resonance energy transfer processes: from tryptophan of...

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Bibliographic Details
Main Authors: Alexander S. Goryashchenko, Maria G. Khrenova, Anna A. Bochkova, Tatiana V. Ivashina, Leonid M. Vinokurov, Alexander P. Savitsky
Format: Article
Language:English
Published: MDPI AG 2015-07-01
Series:International Journal of Molecular Sciences
Subjects:
FRET-sensor
caspase-3
terbium
fluorescent proteins
apoptosis
Online Access:http://www.mdpi.com/1422-0067/16/7/16642

Internet

http://www.mdpi.com/1422-0067/16/7/16642

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