Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies

Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies

A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies—IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories <i>Clearcoli</i...

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Bibliographic Details
Main Authors: Jose Vicente Carratalá, Laia Gifre-Renom, Ramon Roca-Pinilla, Antonio Villaverde, Anna Arís, Elena Garcia-Fruitós, Julieta María Sánchez, Neus Ferrer-Miralles
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:International Journal of Molecular Sciences
Subjects:
inclusion bodies
affinity chromatography
dynamic light scattering
the center of spectral mass
circular dichroism
protein conformers
Online Access:https://www.mdpi.com/1422-0067/22/6/3020

Internet

https://www.mdpi.com/1422-0067/22/6/3020

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