Comparison of Three In-House Real PCR Assays Targeting Kinetoplast DNA, the Small Subunit Ribosomal RNA Gene and the Glucose-6-Phosphate Isomerase Gene for the Detection of <i>Leishmania</i> spp. in Human Serum

Comparison of Three In-House Real PCR Assays Targeting Kinetoplast DNA, the Small Subunit Ribosomal RNA Gene and the Glucose-6-Phosphate Isomerase Gene for the Detection of <i>Leishmania</i> spp. in Human Serum

To perform PCR from serum for the diagnosis of visceral leishmaniasis is convenient and much less invasive than the examination of deeper compartments such as bone marrow. We compared three <i>Leishmania</i>-specific real-time PCRs with three different molecular targets (kinetoplast DNA,...

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Bibliographic Details
Main Authors: Konstantin Tanida, Carsten Balczun, Andreas Hahn, Alexandra Veit, Beatrice Nickel, Sven Poppert, Patrick Leander Scheid, Ralf Matthias Hagen, Hagen Frickmann, Ulrike Loderstädt, Egbert Tannich
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Pathogens
Subjects:
leishmania
visceral
Kala Azar
real-time PCR
test comparison
in-house
Online Access:https://www.mdpi.com/2076-0817/10/7/826

Internet

https://www.mdpi.com/2076-0817/10/7/826

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