A unified censored normal regression model for qPCR differential gene expression analysis.

Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is considered as the gold standard for accurate, sensitive, and fast measurement of gene expression. Prior to downstream statistical analysis, RT-qPCR fluorescence amplification curves are summarized into one single value, the qu...

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Bibliographic Details
Main Authors: Peter Pipelers, Lieven Clement, Matthijs Vynck, Jan Hellemans, Jo Vandesompele, Olivier Thas
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5560691?pdf=render