Efficient Cryopreservation of <i>Populus tremula</i> by In Vitro-Grown Axillary Buds and Genetic Stability of Recovered Plants
Axillary buds of in vitro microshoots were successfully frozen at –196 °C by the one-step freezing method using the protective vitrification solution 2 (PVS2). Microshoots were taken from 11 transgenic lines and three wild type lines. Influence of different explant pretreatments were analyzed from t...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-01-01
|
Series: | Plants |
Subjects: | |
Online Access: | https://www.mdpi.com/2223-7747/10/1/77 |